Nanostructures and Osteoblast Behavior of Thermal Sprayed Calcium Phosphate Splats
K.A. Khor, H. Li, P. Cheang
AZojomo
Abstract
This study aimed to characterize the nanostructures in individual calcium phosphate (CP) splats deposited by thermal spray processes. The CP splats were fabricated using both plasma spraying and high velocity oxy-fuel (HVOF) techniques. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) were employed for observing the nanostructures. A nanostructure with ~ 30nm grains within the CP splats was revealed. This is consistent with the nanostructures exhibited within the CP coating. The present results also further confirmed that hydroxyapatite (HA) decomposition mainly occurred within the melted part of the sprayed particles. Furthermore, after 2 days incubation of the splats in the culture medium, the osteoblast cells have already very well attached and proliferated on the coating. The nanostructures are capable of enhancing the proliferation of the osteoblast cells.
Key words: Hydroxyapatite; Nanostructures; Cell culture; Splats
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